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zetaprobe gt membrane  (Bio-Rad)


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    Structured Review

    Bio-Rad zetaprobe gt membrane
    Zetaprobe Gt Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/zetaprobe+gt+membrane/Zeta-Probe+GT+Membrane/pm41418758-435-6-9
    Average 96 stars, based on 1711 article reviews
    zetaprobe gt membrane - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Northern Blot:

    Article Title: Mechanism of action of sprG1 -encoded type I toxins in Staphylococcus aureus : from membrane alterations to mesosome-like structures formation and bacterial lysis
    Article Snippet: .. For the northern blot assays, 10 μg of RNAs were separated onto an 8% urea-PAGE gel, and electro-transferred onto a ZetaProbe GT membrane (Bio-Rad) in 0.5x TBE buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA) for 2 h at 25 V. RNAs were cross-linked to the membrane by UV irradiation. .. Specific 32 P probes (see ) were labeled with 0.5 μL [γ 32 P]-ATP (5 μCi) and T4 PNK enzyme, and hybridized overnight on the membranes using ExpressHyb solution (Ozyme).

    Article Title: RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus.
    Article Snippet: 1Institut National de la Santé et de la Recherche Médicale, Bacterial Regulatory RNAs and Medicine UMR_S 1230, Rennes, France.. 2Department of Chemistry and Biochemistry, Bern University, Bern, Switzerland.. 3These authors contributed equally: Marie-Laure Pinel-Marie, Régine Brielle.

    Article Title: Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin–antitoxin systems
    Article Snippet: .. For northern blot analysis, 10 μg of RNA was separated on an 8% urea‐PAGE gel and transferred to a ZetaProbe GT membrane (Bio‐Rad, Marnes‐la‐Coquette, France) using 0.5× TBE buffer (90 m m Tris, 90 m m boric acid, 2 m m EDTA) at 25 V for 2 h. RNA was cross‐linked to the membrane through UV irradiation. ..

    Article Title: Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin-antitoxin systems.
    Article Snippet: .. For northern blot analysis, 10 μg of RNA was separated on an 8% ureaPAGE gel and transferred to a ZetaProbe GT membrane (Bio-Rad, Marnes-la-Coquette, France) using 0.5× TBE buffer (90mM Tris, 90mM boric acid, 2 mM EDTA) at 25V for 2 h. RNA was cross-linked to the membrane through UV irradiation. ..

    Article Title: Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
    Article Snippet: .. For the Northern blot assays, 1 to 10 μg of RNA were separated onto an 8% urea–PAGE gel and electrotransferred onto a ZetaProbe GT membrane (Bio-Rad, Hercules, CA, USA) in 0.5× Tris-borate-EDTA buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA) for 2 h at 25 V. RNA was cross-linked to the membrane by ultraviolet irradiation. .. Specific probes ( ) were labeled with 0.5 μL [γ 32 P]-ATP (5 μCi; PerkinElmer, Waltham, MA, USA) and T4 PNK enzyme and hybridized overnight on the membranes with ExpressHyb solution (Ozyme, Saint-Cyr-l’École, France).

    Membrane:

    Article Title: Mechanism of action of sprG1 -encoded type I toxins in Staphylococcus aureus : from membrane alterations to mesosome-like structures formation and bacterial lysis
    Article Snippet: .. For the northern blot assays, 10 μg of RNAs were separated onto an 8% urea-PAGE gel, and electro-transferred onto a ZetaProbe GT membrane (Bio-Rad) in 0.5x TBE buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA) for 2 h at 25 V. RNAs were cross-linked to the membrane by UV irradiation. .. Specific 32 P probes (see ) were labeled with 0.5 μL [γ 32 P]-ATP (5 μCi) and T4 PNK enzyme, and hybridized overnight on the membranes using ExpressHyb solution (Ozyme).

    Article Title: Plant transcripts with long or structured upstream open reading frames in the NDL2 5' UTR can escape nonsense-mediated mRNA decay in a reinitiation-independent manner.
    Article Snippet: Many eukaryotic transcripts contain upstream open reading frames (uORFs).. Translated uORFs can inhibit the translation of main ORFs by imposing the need for reinitiation of translation.. Translated uORFs can also lead to transcript degradation by the nonsense-mediated mRNA decay (NMD) pathway.

    Article Title: RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus.
    Article Snippet: 1Institut National de la Santé et de la Recherche Médicale, Bacterial Regulatory RNAs and Medicine UMR_S 1230, Rennes, France.. 2Department of Chemistry and Biochemistry, Bern University, Bern, Switzerland.. 3These authors contributed equally: Marie-Laure Pinel-Marie, Régine Brielle.

    Article Title: Phage-encoded small RNA hijacks host replication machinery to support the phage lytic cycle.
    Article Snippet: .. The RNA was transferred to a ZetaProbe GT membrane (Bio-Rad) at 20V overnight at 4 ◦ C in 0.5 X TBE. ..

    Article Title: Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin–antitoxin systems
    Article Snippet: .. For northern blot analysis, 10 μg of RNA was separated on an 8% urea‐PAGE gel and transferred to a ZetaProbe GT membrane (Bio‐Rad, Marnes‐la‐Coquette, France) using 0.5× TBE buffer (90 m m Tris, 90 m m boric acid, 2 m m EDTA) at 25 V for 2 h. RNA was cross‐linked to the membrane through UV irradiation. ..

    Article Title: Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin-antitoxin systems.
    Article Snippet: .. For northern blot analysis, 10 μg of RNA was separated on an 8% ureaPAGE gel and transferred to a ZetaProbe GT membrane (Bio-Rad, Marnes-la-Coquette, France) using 0.5× TBE buffer (90mM Tris, 90mM boric acid, 2 mM EDTA) at 25V for 2 h. RNA was cross-linked to the membrane through UV irradiation. ..


    Article Title: Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
    Article Snippet: .. For the Northern blot assays, 1 to 10 μg of RNA were separated onto an 8% urea–PAGE gel and electrotransferred onto a ZetaProbe GT membrane (Bio-Rad, Hercules, CA, USA) in 0.5× Tris-borate-EDTA buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA) for 2 h at 25 V. RNA was cross-linked to the membrane by ultraviolet irradiation. .. Specific probes ( ) were labeled with 0.5 μL [γ 32 P]-ATP (5 μCi; PerkinElmer, Waltham, MA, USA) and T4 PNK enzyme and hybridized overnight on the membranes with ExpressHyb solution (Ozyme, Saint-Cyr-l’École, France).

    Irradiation:

    Article Title: Mechanism of action of sprG1 -encoded type I toxins in Staphylococcus aureus : from membrane alterations to mesosome-like structures formation and bacterial lysis
    Article Snippet: .. For the northern blot assays, 10 μg of RNAs were separated onto an 8% urea-PAGE gel, and electro-transferred onto a ZetaProbe GT membrane (Bio-Rad) in 0.5x TBE buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA) for 2 h at 25 V. RNAs were cross-linked to the membrane by UV irradiation. .. Specific 32 P probes (see ) were labeled with 0.5 μL [γ 32 P]-ATP (5 μCi) and T4 PNK enzyme, and hybridized overnight on the membranes using ExpressHyb solution (Ozyme).

    Article Title: RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus.
    Article Snippet: 1Institut National de la Santé et de la Recherche Médicale, Bacterial Regulatory RNAs and Medicine UMR_S 1230, Rennes, France.. 2Department of Chemistry and Biochemistry, Bern University, Bern, Switzerland.. 3These authors contributed equally: Marie-Laure Pinel-Marie, Régine Brielle.

    Article Title: Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin–antitoxin systems
    Article Snippet: .. For northern blot analysis, 10 μg of RNA was separated on an 8% urea‐PAGE gel and transferred to a ZetaProbe GT membrane (Bio‐Rad, Marnes‐la‐Coquette, France) using 0.5× TBE buffer (90 m m Tris, 90 m m boric acid, 2 m m EDTA) at 25 V for 2 h. RNA was cross‐linked to the membrane through UV irradiation. ..

    Article Title: Membrane selectivity and pore formation of SprA1 and SprA2 hemolytic peptides from Staphylococcus aureus type I toxin-antitoxin systems.
    Article Snippet: .. For northern blot analysis, 10 μg of RNA was separated on an 8% ureaPAGE gel and transferred to a ZetaProbe GT membrane (Bio-Rad, Marnes-la-Coquette, France) using 0.5× TBE buffer (90mM Tris, 90mM boric acid, 2 mM EDTA) at 25V for 2 h. RNA was cross-linked to the membrane through UV irradiation. ..

    Article Title: Impacts of the Type I Toxin–Antitoxin System, SprG1/SprF1, on Staphylococcus aureus Gene Expression
    Article Snippet: .. For the Northern blot assays, 1 to 10 μg of RNA were separated onto an 8% urea–PAGE gel and electrotransferred onto a ZetaProbe GT membrane (Bio-Rad, Hercules, CA, USA) in 0.5× Tris-borate-EDTA buffer (90 mM Tris, 90 mM boric acid, 2 mM EDTA) for 2 h at 25 V. RNA was cross-linked to the membrane by ultraviolet irradiation. .. Specific probes ( ) were labeled with 0.5 μL [γ 32 P]-ATP (5 μCi; PerkinElmer, Waltham, MA, USA) and T4 PNK enzyme and hybridized overnight on the membranes with ExpressHyb solution (Ozyme, Saint-Cyr-l’École, France).

    DNA Hybridization:


    Agarose Gel Electrophoresis:




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